Antibody response to individual rubella virus proteins in congenital and other rubella virus infections
نویسندگان
چکیده
منابع مشابه
Antibody response to individual rubella virus proteins in congenital and other rubella virus infections.
Serum samples from patients with various forms of rubella virus infection were tested for antibodies to each of three viral structural proteins by radioimmunoprecipitation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. In most sera antibody to E1 protein was the predominant species. Sera from patients with congenital rubella syndrome, however, contained significantly more E2 ant...
متن کاملImpaired cellular immunity to rubella virus in congenital rubella.
Specific cell-mediated immunity (CMI) responses to rubella virus were studied in 12 children with documented congenital rubella syndrome employing a (51)Cr lymphocytotoxicity microassay. Hemagglutination inhibition antibody was detected in 11 of the 12 children, with titers ranging from 1:4 to 1:128. CMI to rubella virus was demonstrated in only 3 of the 11 antibody-positive children. The 12th ...
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BACKGROUND the structural proteins of rubella virus, the capsid protein C and the envelope glycoproteins E1 and E2 were produced in lepidopteran insect cells using baculovirus expression vectors. The C-terminal ends of the corresponding proteins were fused to a polyhistidine tag for easy and gentle purification by metal ion affinity chromatography. OBJECTIVES to investigate the maturation of ...
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A dot immunobinding assay ( DIA) was used for a quantitative and qualitative assay of rubella antibody. Purified antigen and conjugated anti-human immunoglobulin (RAHlg) were prepared. Nitrocellulose paper dotted with the antigen was added to serially diluted sera or blood samples. The reacting antibodies were visualized by a peroxidase system. Development of a colored insoluble substrate ...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1985
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.21.3.449-451.1985